Cloned (Comment) | Organism |
---|---|
expression of His-tagged Smu472 in Escherichiac coli strain BL21(DE3) | Streptococcus mutans |
gene ycbY, DNA and amino acid sequence determination and analysis, expression of His-tagged YcbY in Escherichiac coli strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant Smu472, in presence of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine, hanging drop or sitting drop vapor diffusion method, 16°C, X-ray diffraction structure determination and analysis | Streptococcus mutans |
purified recombinant YcbY, in presence of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine, hanging drop or sitting drop vapor diffusion method, 16°C, X-ray diffraction structure determination and analysis | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY | ? | - |
? | |
additional information | Escherichia coli BW25113 | the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY | ? | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | Streptococcus mutans | - |
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | Escherichia coli | RlmK activity in helix 74 of Escherichia coli 23S rRNA | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | Escherichia coli BW25113 | RlmK activity in helix 74 of Escherichia coli 23S rRNA | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene rlmKL or ycbY | - |
Escherichia coli BW25113 | - |
gene rlmKL or ycbY | - |
Streptococcus mutans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Smu472 from Escherichiac coli strain BL21(DE3) by nickel affinity chromatography | Streptococcus mutans |
recombinant His-tagged YcbY from Escherichiac coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY | Escherichia coli | ? | - |
? | |
additional information | Smu472 does not target G2445, and this nucleotide remains unmodified in Gram-positive rRNAs. Product identification by mass spectrometry | Streptococcus mutans | ? | - |
? | |
additional information | the enzyme also catalyzes the N2-methylation of guanine2445 in 23S rRNA, RlmL activity, reaction of EC 2.1.1.173. Product identification by mass spectrometry | Escherichia coli | ? | - |
? | |
additional information | the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY | Escherichia coli BW25113 | ? | - |
? | |
additional information | the enzyme also catalyzes the N2-methylation of guanine2445 in 23S rRNA, RlmL activity, reaction of EC 2.1.1.173. Product identification by mass spectrometry | Escherichia coli BW25113 | ? | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | - |
Streptococcus mutans | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | RlmK activity in helix 74 of Escherichia coli 23S rRNA | Escherichia coli | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | methylation sites in the peptidyl transferase region of Escherichia coli 23S rRNA. structure, overview | Streptococcus mutans | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | RlmK activity in helix 74 of Escherichia coli 23S rRNA, methylation sites in the peptidyl transferase region of Escherichia coli 23S rRNA. structure, overview | Escherichia coli | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | RlmK activity in helix 74 of Escherichia coli 23S rRNA | Escherichia coli BW25113 | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | RlmK activity in helix 74 of Escherichia coli 23S rRNA, methylation sites in the peptidyl transferase region of Escherichia coli 23S rRNA. structure, overview | Escherichia coli BW25113 | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure comparisons with Streptococcus mutans proteins Smu472 and Smu776, the active site and their folding patterns respectively resemble each other, overview | Escherichia coli |
More | structure comparisosns of Streptococcus mutans proteins Smu472 and Smu776 with Escherichia coli RlmKL protein, the active site and their folding patterns respectively resemble each other, overview | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
bifunctional methyltransferase | - |
Escherichia coli |
m2G2069 methyltransferase | - |
Streptococcus mutans |
ribosomal large subunit methyltransferase | - |
Escherichia coli |
RlmKL | - |
Escherichia coli |
Smu472 | - |
Streptococcus mutans |
ycbY | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli | |
S-adenosyl-L-methionine | - |
Streptococcus mutans |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered, Streptococcus mutans proteins Smu472 and Smu776 are respectively Gram-positive orthologs of the N- and C-halves of YcbY | Escherichia coli |
evolution | the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered, Streptococcus mutans proteins Smu472 and Smu776 are respectively Gram-positive orthologs of the N- and C-halves of YcbY | Streptococcus mutans |
physiological function | the YcbY enzyme is a methyltransferase catalyzing two mechanistically different types of RNA modification. The N-terminal region of YcbY adds the m2G2445 modification, while the C-terminal region of YcbY is responsible for the m7G2069 methylation on the opposite side of the same helix, H74. YcbY enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification | Escherichia coli |